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Xiaoming HU,Jing GUO,Chunling BAI,Zhuying WEI,Li GAO,Tingmao HU,Shorgan BOU,Guangpeng LI
《农业科学与工程前沿(英文)》 2016年 第3卷 第1期 页码 87-96 doi: 10.15302/J-FASE-2016087
关键词: safety of transgenic bicistron gene transfer body transgenic mice muscle development
官春云
《中国工程科学》 2002年 第4卷 第8期 页码 34-39
简要介绍了国外油菜转基因育种情况,包括转除草剂抗性基因,病原菌抗性基因,耐重金属基因, 影响种子贮藏产物基因,影响繁殖特性基因,医药和工业产品基因,以及启动子调节基因等。较详细介绍了湖 南农业大学在Bt毒蛋白基因转化甘蓝型油菜育成抗虫新品系、基因工程雄性不育系、恢复系选育和杂种优势利 用,以及反义FAD2基因转化甘蓝型油菜双低品种提高油酸含量的研究结果;浙江省农科院利用反义PEP基因 转化甘蓝型油菜提高种子含油量的研究结果。
胡炜,朱作言
《中国工程科学》 2016年 第18卷 第3期 页码 105-109 doi: 10.15302/J-SSCAE-2016.03.018
2015年11月19日,美国食品药品监督管理局(FDA)批准了转全鱼生长激素基因(gh)大西洋鲑为第一种可供食用的转基因动物产品,从而取得世界转基因动物育种研究与产业化的历史性突破。经过了长达20年的严格审核,转gh大西洋鲑才获准产业化,不仅充分说明了对包括转基因鱼在内的创新产业的管理决策尤其需要尊重科学规律和客观事实;而且表明基础研究只是产业创新的因素之一,转基因知识的科学普及与企业的积极参与是推动高新技术发展和创新产业形成不可或缺的重要条件;高屋建瓴地制定我国基因改良农业品种市场准入的条例和操作细则已经刻不容缓。
Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG
《农业科学与工程前沿(英文)》 2019年 第6卷 第1期 页码 66-72 doi: 10.15302/J-FASE-2018229
Porcine viral diarrhea is an acute and highly contagious enteric disease of pigs that causes huge economic losses worldwide. Porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) are the main pathogens responsible for piglet viral diarrhea. However, currently there is no specific drug available for the effective treatment of viral diarrhea. Therefore, it is necessary to seek an effective method to diminish PEDV and TGEV infection rates. RNA interference has been applied successfully to inhibit the virus replication. It provides a potential strategy for breeding resistant pigs. In this study, four promoters and four short hairpin RNA (shRNA) vectors with LoxP sites at each end of the selectable marker genes were constructed to target PEDV and TGEV. These vectors were then transfected into porcine fetal fibroblasts, G418 resistant transfectants were confirmed by PCR and transgenic SCNT porcine blastocysts were obtained. These results have paved the way for future production of marker-free transgenic resistant to PEDV and TEGV pigs by SCNT.
关键词: piglet diarrhea RNAi PEDV TGEV transgenic resistance breeding
Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice
Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI
《农业科学与工程前沿(英文)》 2014年 第1卷 第3期 页码 179-184 doi: 10.15302/J-FASE-2014020
关键词: recombinant human butyrylcholinesterase (rhBCHE) human lactoferrin bacterial artificial chromosome (hLF BAC) transgenic mice milk
《农业科学与工程前沿(英文)》 2022年 第9卷 第1期 页码 63-74 doi: 10.15302/J-FASE-2021427
There is a growing demand for high-quality agricultural products and more countries have adopted landscape management by sowing flowering plants in agricultural fields as an important branch of conservation biological control. However, there has been less concern over the interactions and trade-offs between floral plants and entomophagous arthropods. This paper review progress in pollen/nectar feeding habits of entomophagous insects including parasitoids and predators which are important natural enemies of crop pests in agricultural fields. Factors that influence the preference of different guilds of natural enemies are reviewed to guide the selection of flowering plants in conservation biological control practices. Most studies find that floral resources have positive effects on both biological traits of natural enemies and their abundance and diversity, and this is believed to contribute greatly to pest control. Furthermore, the potential impacts of floral resources on crop yields are also discussed with an emphasis on a guild of entomophagous insects that provides both pest control and pollination services.
关键词: ecosystem services / flowering plants / natural enemies / nectar / pollen
Gene-editing in plants no longer requires tissue culture
Yi ZHANG, Michael PALMGREN
《农业科学与工程前沿(英文)》 2020年 第7卷 第2期 页码 229-230 doi: 10.15302/J-FASE-2020330
《环境科学与工程前沿(英文)》 2023年 第17卷 第6期 doi: 10.1007/s11783-023-1669-1
● EE2 photodegradation behavior in the presence of four WWTPs’ DOM was explored.
关键词: Photosensitivity sources 17α-ethinylestradiol Photodegradation Dissolved organic matter Wastewater treatment plants
Chunyan Gu,Xichao Yu,Xiaozhu Tang,Leilei Gong,Jingquan Tan,Yuanjiao Zhang,Huili Zheng,Ze Wang,Chenqian Zhang,Yejin Zhu,Zuojian Zhou,Heming Yu,Kai Xu,Jinao Duan,Xiaosong Gu,Ye Yang,
《工程(英文)》 doi: 10.1016/j.eng.2023.09.007
关键词: Traditional Chinese medicine Lycium barbarum L miR162a Osteoporosis Nuclear receptor corepressor Transgenic plants
Pollution of NPEOs in four municipal sewage treatment plants in the north of China
HOU Shaogang, SUN Hongwen
《环境科学与工程前沿(英文)》 2007年 第1卷 第2期 页码 196-201 doi: 10.1007/s11783-007-0034-0
Pilot plants of membrane technology in industry: Challenges and key learnings
Colin A. Scholes
《化学科学与工程前沿(英文)》 2020年 第14卷 第3期 页码 305-316 doi: 10.1007/s11705-019-1860-x
关键词: membrane gas separation membrane contactors carbon capture pilot plants key learnings
Tracking in urban wastewater treatment plants in a cold region: Occurrence, species and infectivity
《环境科学与工程前沿(英文)》 2022年 第16卷 第9期 doi: 10.1007/s11783-022-1533-8
• Cryptosporidium in WWTPs in a cold region was investigated in different seasons.
关键词: WWTPs Cryptosporidium Occurrence Species Infectivity Low temperature
Microplastics in municipal wastewater treatment plants: a case study of Denizli/Turkey
《环境科学与工程前沿(英文)》 2023年 第17卷 第8期 doi: 10.1007/s11783-023-1699-8
● High amounts of microplastics are released to receiving media from WWTPs.
关键词: Microplastics Wastewater treatment plant Removal efficiency Daily discharge
Energy neutrality potential of wastewater treatment plants: A novel evaluation framework integrating
《环境科学与工程前沿(英文)》 2022年 第16卷 第9期 doi: 10.1007/s11783-022-1549-0
• Framework of indicators was established based on energy efficiency and recovery.
关键词: Wastewater treatment plants Energy neutrality potential Energy efficiency Energy recovery Evaluation framework
Inhibitive effects of three compositae plants on
Weihao ZHANG, Fuqing XU, Wei HE, Xing ZHENG, Chen YANG
《环境科学与工程前沿(英文)》 2009年 第3卷 第1期 页码 48-55 doi: 10.1007/s11783-008-0065-1
关键词:
allelopathy
compositae plants
标题 作者 时间 类型 操作
regions included in a bicistronic vector enhances and stabilizes follistatin gene expressions in both transgeniccells and transgenic mice
Xiaoming HU,Jing GUO,Chunling BAI,Zhuying WEI,Li GAO,Tingmao HU,Shorgan BOU,Guangpeng LI
期刊论文
Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine
Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG
期刊论文
Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice
Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI
期刊论文
FLOWERING PLANTS AND ENTOMOPHAGOUS ARTHROPODS IN THE AGRICULTURAL LANDSCAPE: A PRACTISE-ORIENTED SUMMARY
期刊论文
Photosensitivity sources of dissolved organic matter from wastewater treatment plants and their mediation
期刊论文
Lycium barbarum L.-Derived miR162a Functions on Osteoporosis Through Directly Promoting Osteoblast Formation
Chunyan Gu,Xichao Yu,Xiaozhu Tang,Leilei Gong,Jingquan Tan,Yuanjiao Zhang,Huili Zheng,Ze Wang,Chenqian Zhang,Yejin Zhu,Zuojian Zhou,Heming Yu,Kai Xu,Jinao Duan,Xiaosong Gu,Ye Yang,
期刊论文
Pollution of NPEOs in four municipal sewage treatment plants in the north of China
HOU Shaogang, SUN Hongwen
期刊论文
Tracking in urban wastewater treatment plants in a cold region: Occurrence, species and infectivity
期刊论文
Energy neutrality potential of wastewater treatment plants: A novel evaluation framework integrating
期刊论文